Coding
HRT2

Part:BBa_K1088003:Design

Designed by: Andreas Kjær, Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2013-07-10)

Cis-1,4-prenyltransferase obtained from Hevea brasiliensis cDNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized codons to fit the most used codons of E. coli.


Source

The gene was synthesized by GenScript cDNA source(1) Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis : Kasem Asawatreratanakul, Seiji Takahashi, Yuan-Wei Zhang, Atiya Rattanapittayaporn, Dhirayos Wititsuwannakul, Rapepun Wititsuwannakul, and Tanetoshi Koyama

References